AN ANAPLASMA-CENTRALE DNA PROBE THAT DIFFERENTIATES BETWEEN ANAPLASMA-OVIS AND ANAPLASMA-MARGINALE DNA

作者：VISSER ES^*; AMBROSIO RE; DEWAAL DT

来源：Veterinary Microbiology, 1991, 28(3): 313-325.

DOI：10.1016/0378-1135(91)90086-U

An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A. centrale, 0.5 ng A. marginale and 3.9 ng A. ovis DNA. The hybridisation pattern obtained with pAC5-12 on digests of A. centrale, A. marginale and A. ovis DNA suggests that this probe detects EcoR1 and Hind111-polymorphisms. Probe pAC5-12 could detect A. ovis DNA in 36% of blood samples tested compared to the 33% detectability obtained with microscopy.

• 出版日期1991-8-15