Antioxidant activity and phenolic compounds of buckwheat and barley by the data of spectrophotometry and HPLC

作者：Aleksenko S S^*

来源：Journal of Analytical Chemistry, 2013, 68(5): 458-465.

DOI：10.1134/S106193481305002X

• buckwheat
• barley
• antioxidant activity
• 1,1-diphenyl-2-picrylhydrazyl
• Folin-Ciocalteu reagent
• phenolic compounds
• flavan-3-ols
• proanthocyanidins
• HPLC with UV detection
• HPLC-electrospray ionization mass spectrometry

The antioxidant activity of buckwheat and barley extracts by reaction with 1,1-diphenyl-2-picrylhydrazyl and the total of phenolic compounds have been determined using the Folin-Ciocalteu reagent. It has been found that water-ethanol extracts of buckwheat are characterized by higher antioxidant activity (6.2 +/- 0.5 mu M-eq. of Trolox/g) and concentration of phenolic compounds (4.41 +/- 0.07 mg-eq. of rutin/g) compared to barley extracts (4.2 +/- 0.3 mu M-eq. of Trolox/g and 2.4 +/- 0.1 mg-eq. of rutin/g, respectively). A series of phenolic compounds have been identified by HPLC with UV detection and mass spectrometric detection with electrospray ionization. The main phenolic compounds-antioxidants in buckwheat extracts are rutin, catechin and epicatechin, 1-O-caffeoyl-O-rutinoside (m/z 487), and epicatechin-O-3,4-dimethylgallate (m/z 469), and in the barley extract, catechin, prodelphinidin B3 (m/z 593), procyanidin B3 (m/z 577), and procyanidin C2 (m/z 865).

• 出版日期2013-5