A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodes

作者：Welle Theresa M; Alanis Kristen; Colombo Michelle L; Sweedler Jonathan V; Shen Mei^*

来源：Chemical Science, 2018, 9(22): 4937-4941.

DOI：10.1039/c8sc01131a

• statistical manifold
• phi-family
• information geometry
• exponential arcs
• mixture arcs

Extra-synaptic exocytosis is an essential component of cellular communication. A knowledge gap exists in the exocytosis of the non-redox active transmitter acetylcholine. Using the nano-interface between two immiscible electrolyte solutions and scanning electrochemical microscopy (SECM), a high resolution spatiotemporal study of acetylcholine exocytosis is shown from an individual neuronal soma. The nanoelectrode was positioned similar to 140 nm away from the release sites on the soma using an SECM. The quantitative study enables the obtaining of key information related to cellular communication, including extracellular concentration of the neurotransmitter, cellular permeability, Ca2+ dependence on somatic release, vesicle density, number of molecules released and the release dynamics. Measurements were achieved with a high signal to noise ratio of 6-19. The released neurotransmitter with a concentration of 2.7 (+/- 1.0) mu M was detected at the nanoelectrodes with radii of 750 nm to 860 nm.

• 出版日期2018-6-14