Natural variation in folate levels among tomato (Solanum lycopersicum) accessions

作者：Upadhyaya Pallawi; Tyagi Kamal; Sarma Supriya; Tamboli Vajir; Sreelakshmi Yellamaraju; Sharma Rameshwar^*

来源：Food Chemistry, 2017, 217: 610-619.

DOI：10.1016/j.foodchem.2016.09.031

• Folate
• Solanum lycopersicum
• Natural accessions
• Single nucleotide polymorphism
• EcoTILLING

Folate content was estimated in tomato (Solarium lycopersicum) accessions using microbiological assay (MA) and by LC-MS. The MA revealed that in red-ripe fruits folate levels ranged from 4 to 60 mu g/100 g fresh weight. The LC-MS estimation of red-ripe fruits detected three folate forms, 5-CH3-THF, 5-CHO-THF, 5,10-CH+THF and folate levels ranged from 14 to 46 mu g/100 g fresh weight. In mature green and red ripe fruit, 5-CH3-THF was the most abundant folate form. Comparison of LC-MS with MA revealed that MA inaccurately estimates folate levels. The accumulation of folate forms and their distribution varied among accessions. The single nucleotide polymorphism was examined in the key genes of the folate pathway to understand its linkage with folate levels. Despite the significant variation in folate levels among tomato accessions, little polymorphism was found in folate biosynthesis genes. Our results indicate that variation in folate level is governed by a more complex regulation at cellular homeostasis level.

• 出版日期2017-2-15