Anticancer and anti-angiogenesis effect of the leaf extract of Sargassum wightii against osteosarcoma cancer cells

作者：Yu, Qi-Long; Tang, Zhao-Chun^*; Li, Jin-Ge; Wang, Guo-Liang; Wang, Pei-Jun; Pan, Zhao-Bao

来源：Bangladesh Journal of Pharmacology, 2015, 10(2): 351-357.

DOI：10.3329/bjp.v10i2.22473

• Anti-angiogenic
• Anti-osteosarcoma
• Sargassum wightii

This study was aimed to evaluate the chemical composition of ethanolic leaf extract of a marine macro-alga Sargassum wightii (ELESW) followed by its vascular endothelial growth factor (VEGF) induced anti-angiogenic effect against human umbilical vein endothelial cells (HUVECs). The effect of ELESW on cell viability and its potential against in vivo angiogenesis was assessed by using the MTT and CAM assay respectively. The phenolic, flavonoid, polysaccharides, and protein content of ELESW were found to be 56.0 +/- 4.1 mg GAE/g, 26.3 +/- 3.6 mg QE/g, 284.2 +/- 6.2 mu g/mL, 100.9 +/- 8.9 mu g/mL, respectively. In angiogenesis, ELESW (100, 500 and 1,000 mu g/mL) significantly inhibited VEGF induced angiogenesis as well as VEGF-induced proliferation and tube formation of HUVECs in a dose-dependent manner. Upon stimulition by VEGF, HUVECs rapidly increased reactive oxygen species production, which was significantly blocked by treatment with ELESW. These findings suggesting that S. wightii could be a valuable candidate of therapeutic efficacy.

• 出版日期2015
• 单位潍坊市人民医院