Antitumor effect of the antimicrobial peptide GLI13-8 derived from domain of the avian -defensin-4

作者：Gu, Yao; Dong, Na; Shan, Anshan^*; Ma, Qingquan; Li, Jianping; Cheng, Baojing

来源：Acta Biochimica et Biophysica Sinica, 2013, 45(11): 904-911.

DOI：10.1093/abbs/gmt098

• antimicrobial peptide
• AvBD-4 derivative
• carcinoma
• apoptosis

We previously reported that GLI13-8, one of cationic antimicrobial peptides from linear avian -defensin-4 (RL38) analogs, exhibited high antimicrobial activities against both Gram-negative and Gram-positive bacteria. In the present study, we reported the in vitro cytotoxicity of GLI13-8 using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results showed that the cytotoxicity of GLI13-8 in three human carcinoma cells (HepG2, SGC7901, and A375) was in a dose-dependent manner. When the concentration of GLI13-8 is 128 M, it had no toxicity towards the normal human fibroblasts (MRC-5). The Annexin-V-FITC/PI staining assay, the Hoechst 33258/PI staining assay, the permeability of fluorescein macromolecules and scanning electron microscope assays, mitochondrial membrane potential assay, caspases-3 and poly ADP-ribose polymerase (PARP) assays have been carried out. Results indicated that apoptosis was induced by GLI13-8 in HepG2 cells, and demonstrated that GLI13-8 induced loss of mitochondrial membrane potential, disruption of HepG2 cell membranes, and activation of caspase-3 and PARP. These findings suggested that GLI13-8 may be an effective agent for HepG2 cells.

• 出版日期2013-11
• 单位东北农业大学