INFLUENCE OF HYDROCOLLOIDS OF Ag, Au, AND Ag/Cu ALLOY NANOPARTICLES ON THE INFLAMMATORY STATE AT TRANSCRIPTIONAL LEVEL

作者：Sawosz Ewa^*; Grodzik Marta; Lisowski Pawel; Zwierzchowski Lech; Niemiec Tomasz; Zielinska Marlena; Szmidt Maciej; Chwalibog Andre

来源：Bulletin of the Veterinary Institute in Pulawy, 2010, 54(1): 81-85.

• chicken embryo
• nanoparticle
• gene expression
• NF-kappa B
• inflammation
• therapy

The objective of this investigation was to evaluate the pro- or anti-inflammatory properties of nanoparticles of Ag, Au, and Ag/Cu alloy by examining the expression of NF-kappa B mRNA. The experiment was performed in ovo, on the chicken embryos' model. The nanoparticles had no effect on embryos' survival; the embryos from all groups were properly developed, without any abnormalities. Contrary to Ag and Au, nanoparticles of Au/Cu increased NE-kappa B mRNA expression in embryo liver, indicating a proinflammatory effect. After treatment with LPS there was a significant decrease in NE-kappa B mRNA expression in the liver of embryos treated with Ag, compared to the placebo. Au, and Ag/Cu groups, indicating that Ag nanoparticles act as a potential anti-inflammatory factor. The results indicate the lack of influence of Ag and Au nanoparticles on NE-kappa B mRiNA expression in chicken embryo liver. Contrary to Ag and Au, nanoparticles of Ag/Cu alloy may be considered as a pro-inflammatory factor. Nanoparticles of Ag, but not Au and Ag/Cu, can prevent over-expression of NE-kappa B mRNA after LPS stimulation.

• 出版日期2010