Analysis and purification of O-decanoyl sucrose regio-isomers by reversed phase high pressure liquid chromatography with evaporative light scattering detection

作者：Ritthitham Sinthuwat; Wimmer Reinhard; Sten**alle Allan; Pedersen Lars Haastrup^*

来源：Journal of Chromatography A, 2009, 1216(25): 4963-4967.

DOI：10.1016/j.chroma.2009.04.054

• Sucrose monodecanoate
• Regio-isomers
• Acyl migration
• RP-HPLC
• ELSD

Purification of seven regio-isomers of O-decanoyl sucrose, 2-O-, 3-O-, 4-O, 6-O-, 3'-O, 4'-O- and 6'-O-decanoyl sucrose, were performed by LC followed by preparative RP-HPLC with ELSD. Using an optimized gradient of acetonitrile in water 2-O-, 3-O, 6-O- and 3'-O-decanoyl sucrose were purified in yields (w/w) of 52.5%, 34.7%, 45.0% and 36.9%, respectively. In the purified preparations of the 2-O- and 3'-O-decanoyl sucrose respectively, acyl migration was observed as a result of the drying process. Lyophilization resulted in the highest purities (w/w) of 96% and 100% for the 2-O- and the 3'-O-decanoyl sucrose, respectively.

• 出版日期2009-6-19