A Sensitive Method for Determination Glycolic Acid, Mono- and Di-Chloroacetic Acids in Betaine Media Using Amino-Functionalized SBA-15 as a Sorbent and HPLC Assay

作者：Soleimani Majid^*; Khani Azam; Moazzen Elahe; Ebrahimzadeh Homeira; Samiei Azam; Masoomi Leila

来源：Chromatographia, 2013, 76(1-2): 33-40.

DOI：10.1007/s10337-012-2366-6

• High performance liquid chromatography
• Solid-phase extraction
• Amino-modified nanoporous silica
• Betaine
• Mono-and dichloroacetic acid
• Glycolic acid

In this paper, amino-modified nanoporous silica (APS-SBA-15) was synthesized as a new solid-phase sorbent for the extraction of glycolic acid, monochloroacetic acid, and dichloroacetic acid in synthetical betaine products. Octadecyl silica cartridge was used to reduce the concentration of matrix betaine. PS-Ag+ pre-treatment cartridge was applied to remove high Cl- concentration. The obtained effluent sample was passed through of the APS-SBA-15 sorbent. The effect of pH, flow rate of sample and eluent, and type and volume of the eluent were investigated and optimized. Chloroacetate and glycolate were eluted with 0.8 mol L-1 solution of HClO4 and measured by HPLC with a UV-vis detector. At optimum effective parameters, preconcentration factor of 129 was achieved in this method. The detection limits of mono- and dichloroacetic acid and glycolic acid were 13, 3.7, and 8.6 ng L-1, respectively.

• 出版日期2013-1