Leucobacter ruminantium sp nov., isolated from the bovine rumen

作者：Chun Byung Hee; Lee Hyo Jung; Jeong Sang Eun; Schumann Peter; Jeon Che Ok^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2017, 67(8): 2634-2639.

DOI：10.1099/ijsem.0.002003

• Leucobacter ruminantium
• actinobacteria
• new taxa
• bovine rumen

A Gram-stain-positive, lemon yellow-pigmented, non-motile, rod-shaped bacterium, designated strain A2(T), was isolated from the rumen of cow. Cells were catalase-positive and weakly oxidase-positive. Growth of strain A2(T) was observed at 25-45 degrees C (optimum, 37-40 degrees C), at pH 5.5-9.5 (optimum, pH 7.5) and in the presence of 0-3.5% (w/v) NaCl (optimum, 1 %). Strain A2(T) contained iso-C-16 (: 0) and anteiso-C-15 : 0 as the major cellular fatty acids. Menaquinone-11 was detected as the sole respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2(T) formed a distinct phyletic lineage within the genus Leucobacter. Strain A2(T) was most closely related to 'Leucobacter margaritiformis' A23 (97.7% 16S rRNA gene sequence similarity) and Leucobacter tardus K 70/01(T) (97.2 %). The major polar lipids of strain A2(T) were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. Strain A2(T) contained a B-type cross-linked peptidoglycan based on 2,4-diaminobutyric acid as the diagnostic diamino acid with threonine, glycine, alanine and glutamic acid but lacking 4-aminobutyric acid. The G+C content of the genomic DNA was 67.0 %. From the phenotypic, chemotaxonomic and molecular features, strain A2(T) was considered to represent a novel species of the genus Leucobacter, for which the name Leucobacter ruminantium sp. nov. is proposed. The type strain is A2(T) (=KACC 17571(T) =JCM 19316(T)).

• 出版日期2017-8