Axonal protection by thioredoxin-1 with inhibition of interleukin-1 beta in TNF-induced optic nerve degeneration

作者:Kitaoka Yasushi*; Tanito Masaki; Kojima Kaori; Sase Kana; Kaidzu Sachiko; Munemasa Yasunari; Takagi Hitoshi; Ohira Akihiro; Yodoi Junji
来源:Experimental Eye Research, 2016, 152: 71-76.
DOI:10.1016/j.exer.2016.09.007

摘要

Interleukin (IL)-1 beta, a proinflammatory cytokine, is a key mediator in several acute and chronic neurological diseases. Thioredoxin-1 (TRX1) acts as an antioxidant and plays a protective role in certain neurons. We examined whether exogenous TRX1 exerts axonal protection and affects IL-1 beta levels in tumor necrosis factor (TNF)-induced optic nerve degeneration in rats. Immunoblot analysis showed that 1L-1 beta was upregulated in the optic nerve after intravitreal injection of TNF. Treatment with recombinant human (rh) TRX1 exerted substantial protective effects against TNF-induced axonal loss. The increase in the IL-1 beta level in the optic nerve was abolished by rhTRX1. Treatment with rhTRX1 also significantly inhibited increased glial fibrillary acidic protein (GFAP) levels induced by TNF. Immunohistochemical analysis showed substantial colocalization of IL-1 beta and GFAP in the optic nerve after TNF injection. These results suggest that IL-1 beta is upregulated in astrocytes in the optic nerve after TNF injection and that exogenous rhTRX1 exerts axonal protection with an inhibitory effect on IL-1 beta.

  • 出版日期2016-11