Assessing refrigerating and freezing effects on the biological/chemical composition of two livestock manures

作者：Pratt Chris^*; Redding Mathew; Hill Jaye; Mudge Stephen Rowland; Westermann Maren; Paungfoo Lonhienne Chanyarat; Schmidt Susanne

来源：Agriculture Ecosystems & Environment, 2014, 197: 288-292.

DOI：10.1016/j.agee.2014.08.012

• BMP
• FISH
• Freezing
• GHG
• Livestock manure
• Nutrient
• Refrigeration
• Storage

Assessing storage impacts on manure properties is relevant to research associated with nutrient-use efficiency and greenhouse gas (GHG) emissions. We examined the impact of cold storage on physicochemical properties, biochemical methane-emitting potential (BMP) and the composition of microbial communities of beef feedlot manure and poultry broiler litter. Manures were analysed within 2 days of collection and after 2 and 8 weeks in refrigerated (4 degrees C) or frozen (-20 degrees C) storage. Compared with fresh manure, stored manures had statistically significant (p < 0.05) but comparatively minor (<10%) changes in electrical conductivity, chloride and ammonium concentrations. Refrigeration and freezing did not significantly affect (p > 0.05) BMP in both manure types. We did not detect ammonium-or nitrite-oxidising bacterial taxa (AOB, NOB) using fluorescence in situ hybridisation (FISH). Importantly, the viability of microbes was unchanged by storage. We conclude that storage at -20 degrees C or 4 degrees C adequately preserves the investigated traits of the studied manures for research aimed at improving nutrient cycling and reducing GHG emissions.

• 出版日期2014-12-1