科研之友
微信
新浪微博
Facebook
分享链接
摘要
An analytical method based on ultra performance liquid chromatography tandem mass spectrometry has been developed for the simultaneous determination of 6 statins residues in Chicken and egg. The statins were analyzed on an ACQUITY UPLC BEH C-18 column(100 mm x 2.1 mm, 1.7 mu m), using the methanol -5 mmol ammonium acetate (added 0.1% formic acid) as the mobile phase and a gradient elution program with a cycle time of 20 min, detected by electrospray ionization mass spectrometry in both positive and negative mode with multiple reaction monitoring, quantified by external standard method. The limits of detection (LOD, S/N = 3) and quantification (LOQ, S/N = 10) of 6 statins were 0.1 - 0.5 mu g/kg and 0.3 - 2.0. mu g/kg, respectively. The correlation coefficients of linear calibration curve were over 0.9910 in the ranges of 1.0 - 100 mu g/kg. Average fortified recoveries in Chicken and egg samples were 80.9% - 97.2% and 79.0% - 87.2% at the spiked level of 20 mu g/kg, and the relative standard deviations were 1.3% - 10.2% and 1.5% - 10.7%, respectively.
- 出版日期2014
- 单位中国农业大学
