Apoferritin as a bionanomaterial to facilitate the electron transfer reactivity of hemoglobin and the catalytic activity towards hydrogen peroxide

作者：Chen Guifang; Zhu Xiaoli; Meng Fanben; Yu Zhiguo; Li Genxi^*

来源：Bioelectrochemistry, 2008, 72(1): 77-80.

DOI：10.1016/j.bioclechem.2007.11.005

• apoferritin
• bionanomaterial
• hemoglobin
• hydrogen peroxide

In this report, apoferritin as a stable bionanomaterial was modified with hemoglobin on pyrolytic graphite electrode. Rapid electron transfer reactions of hemoglobin were achieved with the help of apoferritin in a large pH range. Moreover, hemoglobin as an enzyme exhibits fine electrocatalytic activity towards the reaction of hydrogen peroxide, and a wide concentration range of linear relationship between the reduction peak current and the concentration of hydrogen peroxide has been obtained with a higher upper detection limit, which may be further developed for a hydrogen peroxide biosensor. Therefore, a new property of apoferritin is explored, in which apoferritin works as a bionanomaterial to be an accelerant of the electron transfer of Hb and a stabilizer to retain the catalytic ability of the protein under mal-condition.

• 出版日期2008-2
• 单位上海大学; 南京大学