A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout

作者：Erard Nicolas; Knott Simon R V^*; Hannon Gregory J^*

来源：Molecular Cell, 2017, 67(2): 348-+.

DOI：10.1016/j.molcel.2017.06.030

We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.

• 出版日期2017-7-20