Downregulation of circ_008018 protects against cerebral ischemia-reperfusion injury by targeting miR-99a

作者：Yang, Xuelian; Ji, Haifeng; Yao, Yulan; Lai, Xiaoyin; Jiang, Yufeng; Wu, Dayu; Cai, Liying; Zhu, Wei; Gu, Xiaju; Hu, Rongguo; Li, Longxuan; Xu, Lijuan^*; Jiang, Mei^*

来源：Biochemical and Biophysical Research Communications, 2018, 499(4): 758-764.

DOI：10.1016/j.bbrc.2018.03.218

• Circular RNAs
• circ_008018
• Cerebral ischemia-reperfusion injury
• miR-99a

Circular RNAs (circRNAs) are highly expressed in eukaryotic cells and regulate physiological and pathophysiological processes. However, the role of circRNAs in cerebral ischemia-reperfusion (I/R) injury remains largely unknown. In this study, we found that circ_008018 level was higher in the cortical tissue of mice with middle cerebral artery occlusion as compared to those in the sham group 24 h after reperfusion. Knockdown of circ_008018 attenuated cerebral I/R-induced brain tissue damage and neurological deficits in mice by inducing microRNA miR-99a overexpression. The decreased phosphorylation of Akt and glycogen synthase kinase 3 beta caused by I/R was partly reversed by circ_008018 silencing or miR-99a overexpression. Taken together, these results provide new insight into the mechanisms of apoptosis resulting from cerebral I/R injury and suggest that targeted inhibition of circ_008018 can protect against subsequent neurological damage.

• 出版日期2018-5-23
• 单位上海市浦东新区公利医院; 中国人民解放军第二军医大学; 安徽医科大学