Microbial community analysis of perchlorate-reducing cultures growing on zero-valent iron

作者：Son Ahjeong^*; Schmidt Carl J; Shin Hyejin; Cha Daniel K

来源：Journal of Hazardous Materials, 2011, 185(2-3): 669-676.

DOI：10.1016/j.jhazmat.2010.09.070

• Perchlorate
• Zero-valent iron
• FAME
• Chlorite dismutase (cld) gene
• Phylogenetic analysis

Anaerobic microbial mixed cultures demonstrated its ability to completely remove perchlorate in the presence of zero-valent iron. In order to understand the major microbial reaction in the iron-supported culture, community analysis comprising of microbial fatty acids and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) techniques was performed for perchlorate reducing cultures. Analysis of fatty acid methyl esters (FAMEs) and subsequent principal component analysis (PCA) showed clear distinctions not only between iron-supported perchlorate reducing culture and seed bacteria, but also among perchlorate-reducing cultures receiving different electron donors. The DGGE pattern targeting the chlorite dismutase (cld) gene showed that iron-supported perchlorate reducing culture is similar to hydrogen-fed cultures as compared to acetate-fed culture. The phylogenetic tree suggested that the dominant microbial reaction may be a combination of the autotrophic and heterotrophic reduction of perchlorate. Both molecular and chemotaxonomic experimental results support further understanding in the function of zero-valent iron as an adequate electron source for enhancing the microbial perchlorate reduction in natural and engineered systems.

• 出版日期2011-1-30