A combination of interdisciplinary analytical tools for evaluation of multi-layered coatings on medical grade stainless steel for biomedical applications

作者：Maver Uros^*; Xhanari Klodian; Zizek Marko; Korte Dorota; Gradisnik Lidija; Franko Mladen; Finsgar Matjaz^*

来源：European Journal of Pharmaceutics and Biopharmaceutics, 2018, 128: 230-246.

DOI：10.1016/j.ejpb.2018.05.002

• Multi-layered coatings
• Biomedical application
• Drug release
• Thermal diffusivity and conductivity analysis
• Photothermal beam deflection spectroscopy
• Electrochemical impedance spectroscopy
• AFM
• ATR-FTIR
• Biocompatibility
• Method validation

In this comprehensive study several analytical techniques were used in order to evaluate multi-layered biomedical surface coatings composed of a drug (diclofenac) and a polymer (chitosan). Such a thorough examination is of paramount importance in order to assure safety and prove efficiency of potential biomedical materials already at the in vitro level, hence leading to their potentially faster introduction to clinical trials.
For the first time a novel technique based on thermal diffusivity and conductivity measurements (photothermal beam deflection spectroscopy - BDS) was employed in order to analyse in a non-destructive way the thickness of respective layers, together with their thermal diffusivity and conductivity. In addition to attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), BDS confirmed successive surface layers of the prepared coatings. Scanning electron microscopy and atomic force microscopy were used to examine structural information on the macro- and micro/nano-scale, respectively. Surface hydrophobicity was measured with the contact angle analysis, which clearly showed differences in hydrophobicity between coated and noncoated samples. Considering the targeted application of the prepared coatings (as implant in orthopaedic treatments), the in vitro drug release was analysed spectrophotometrically to examine the coatings potential for a controlled drug release. Furthermore, the material was also tested by electrochemical impedance spectroscopy and cyclic polarisation techniques, which were able to detect even minor differences between the performance of the coated and non-coated materials. As the final test, the biocompatibility of the coatings with human osteoblasts was determined.

• 出版日期2018-7