科研之友
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摘要
A novel method was developed for assaying affinity of aptamer with target protein and detecting the protein, which was based on the specific recognition of aptamers toward targets and high sensitivity of colloidal gold colorimetric. Oligonucleotide aptamer can protect colloid gold from coagulation in high salt conditions. When the aptamer bound to the target protein, the naked gold nanoparticles aggregated and the color of the solution changed. Then the affinity of target protein with aptamer and the concentration of target protein could be detected by monitoring A(520) value. The best detection system was established, while the principal influence factors, such as the amount of aptamer and target protein, incubation time, competitive reaction time and the concentration of sodium chloride, were optimized.
- 出版日期2015-4-10
- 单位河北大学
