Adipogenic Differentiation of Feeder Cells by Human Embryonic Stem cells

作者：Lee Sunray; Park Hyun Sook^*

来源：Tissue Engineering and Regenerative Medicine, 2010, 7(1): 82-86.

• human embryonic stem cells
• adipogenesis
• feeders
• mouse embryonic fibrobalsts
• human skin fibroblasts
• human mesenchymal stem cells

Human embryonic stem(hES) cells have generally been cultured on mouse embryonic fibroblast feeder cells. Paracrine factors from feeder cells have drawn Much attention in their relation to the maintenance of self-renewal and pluripotency of hES cells. There has however been no attention on factors from hES cells and their effects on feeder cells. In the present study, we examined the effect of hES cells on feeder cells, mouse embryonic fibroblasts, human mesenchymal stem cells and human skin fibroblasts, in indirect co-culture system using porous membrane. We observed lipid droplet formation in feeder cells as early as 2 days after co-culture and lipid vacuole matured with time in the influence of hESCs. Only with hES-conditioned medium adipogeneisis of feeder cells occurred but co-culture with hES cells dramatically increased adipogenesis of feeder cells. This implied adipogenesis of feeder cells occurred via paracrine communication between hES cells and feeder cells. Further investigation on how adipogenesis of feeder cells affects the niche Of human embryonic stem cells is required.

• 出版日期2010-3