摘要
A sensitive and specific HPLC-UV method was developed and validated for the determination of xyloketal B in rat plasma. Following liquid-liquid extraction, the separation was performed using an isocratic mobile phase of methanol-acetonitrile-water (30/30/40, v/v/v) on a Phenomenex C-18 column (4.6mm x 250 mm,5 mu m). The eluent was monitored at 220 nm and at a flow rate of 0.8 ml min(-1). A linear curve over the concentration range of 1-128 mu g/m1(r > 0.999) was established. The LLOQ of the method was 1 mu g/ml. Good precision and accuracy at concentrations of 2.5, 25 and 100 mu g/ml were obtained. The recovery of xyloketal B in plasma was > 87.91%. The validated method was found to be specific, precise and accurate in the study. The analytic method was satisfactorily applied to perform preclinical pharmacokinetic study of xyloketal B in rat plasma.