Protection of Prenylated Flavonoids from Mori Cortex Radicis (Moraceae) against Nitric Oxide-induced Cell Death in Neuroblastoma SH-SY5Y Cells

作者：Lee Hak Ju; Lyu Da Hyun; Koo Uk; Nam Kung Woo; Hong Seong Su; Kim Kem Ok; Kim Kyeong Ho; Lee Dongho; Mar Woongchon^*

来源：Archives of Pharmacal Research, 2012, 35(1): 163-170.

DOI：10.1007/s12272-012-0118-7

• Mori cortex radicis
• Prenylated flavonoids
• Nitric oxide
• Neurodegenerative disease

Seven prenylated flavanoids, licoflavone C (1), cyclomulberrin (2), neocyclomorusin (3), sanggenon I (4), morusin (5), kuwanon U (6) and kuwanon E (7), and three 2-arylbenzofurans, moracin P (8), moracin O (9), and mulberrofuran Q (10) were isolated from the MeOH extract of Mori Cortex Radicis. Among these, compounds 2-7 enhanced cell viability in a dose-dependent manner against sodium nitroprusside-induced cell death in neuroblastoma SH-SY5Y cells, which was measured by MTT reduction assay (EC50 values of 4.4, 5.6, 8.0, 6.4, 8.7, and 11.9 mu g/mL, respectively). Among 10 compounds, C-3 prenylated flavones (2, 3, and 5) and prenylated flavanones (4, 6, and 7) showed cell protection. However, compound 1 which lacks the prenyl group at C-3 and three 2-arylbenzofurans (8-10) did not show protective effect. The order of cell protection was as follow: C-3 prenylated flavones (2, 3, and 5) %26gt; prenylated flavanones (4, 6, and 7) %26gt; 2-arylbenzofurans (8-10) and flavone (1). From this result, we show that some prenylated flavones and flavanones might protect neuronal cells against nitrosative stress-mediated cell death. Even though further evaluations are necessary in vitro and in vivo study, we carefully suggest that some prenylated flavonoids from Mori Cortex Radicis might protect neuronal cells from neurodegenerative diseases.

• 出版日期2012-1