New Generation of Ensemble-Decision Aliquot Ranking Based on Simplified Microfluidic Components for Large-Capacity Trapping of Circulating Tumor Cells

作者：Zhao Mengxia; Nelson Wyatt C; Wei Bingchuan; Schiro Perry G; Hakimi Bejan M; Johnson Eleanor S; Anand Robbyn K; Gyurkey Grace S; White Lisa M; Whiting Samuel H; Coveler Andrew L; Chiu Daniel T^*

来源：Analytical Chemistry, 2013, 85(20): 9671-9677.

DOI：10.1021/ac401985r

Ensemble-decision aliquot ranking (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. Here, we report the next generation of eDAR, where we designed and optimized a new hydrodynamic switching scheme for the active sorting step in eDAR, which provided fast cell sorting with an improved reproducibility and stability. The microfluidic chip was also simplified by incorporating a functional area for subsequent purification using microslits fabricated by standard lithography method. Using the reported second generation of eDAR, we were able to analyze 1 mL of whole-blood samples in 12.5 min, with a 95% recovery and a zero false positive rate (n = 15).

• 出版日期2013-10-15