Molecular characterization of plasmid DNA from Weisselia cibatia isolated from kimchi

作者：Park Myeong Soo; Kim Seong Hoon; Kim Jong Doo; Oh Seung Eun; Park Kab Joo; Lee Si Kyung; Ji Geun Eog; Jung Yoon Hee; Kim Soo Young^*

来源：Korean Journal of Genetics, 2007, 29(1): 29-35.

• kimchi
• lactic acid bacteria
• plasmid DNA
• Weissella

Four plasmid-harboring lactic acid bacteria were isolated from kimchi and identified. Among them Weissella sp. KLC140, identified according to 16S rRNA sequence, harbored three plasmids of 1.5 kb (pKLCA), 3.4 kb (pKLCB), and 10.0 kb (pKLCC). Nucleotide sequence analysis of pKLCA (1490 bp, 41.5% G+C) revealed one open reading frame (orfAl), whose predicted product consisted of 258 amino acids and showed similarities to several replication proteins of the rolling circle replication (RCR) type. A conserved tyrosine residue necessary for cleavage at the double strand origin was found in orfA1. A pT181-like double strand origin with three inverted repeats was found downstream of orfA1. Nucleotide sequence analysis of pKLCB (3353 bp, 37.2% G+C) showed two ORFs, orfB1 predicting a 205-amino-acid protein and orfB2 predicting a 152-amino-acid protein. OrfB1 displayed significant sequence similarity "ith cadmium binding proteins from several bacteria. OrfB2 shared homology with acetyltransferases from several bacteria. This is the first report of the isolation and characterization of novel plasmids from the genus Weissella.

• 出版日期2007-3
• 单位安阳工学院