DECREASED PERCENTAGE OF CD4+ CD25+ REGULATORY T CELLS AND GITR GENE EXPRESSION IN THE SPLEEN OF FLUOROSED MALE MICE

作者：Zhang, Guanghe; Han, Tianlong; Wang, Min; Wan, Shuangxiu; Chen, Yan; Wang, Jundong^*

来源：Fluoride, 2017, 50(1): 29-40.

• CD4(+) CD25(+) regulatory T cells
• Fluoride
• GITR gene
• Mice
• Spleen

In view of the important role of the glucorticoid-induced tumor necrosis factor-related receptor (GITR) in the inhibiting function of CD4(+) CD25(+) regulatory T cells, a study was undertaken to evaluate the effects of the fluoride ion (F) on CD4(+) CD25(+) regulatory T cells and the expression of the GITR gene in the spleen in mice. Forty-eight healthy 7-week-old male Kunming mice were divided randomly into four groups and exposed to F at 0 (control), 50, 100, and 150 mg/L NaF in their drinking water for 90 days. At the end of 90 days, the CD4(+) CD25(+) regulatory T cells were measured by flow cytometry (FCM). With the help of quantitative real-time polymerase chain reaction (QRT-PCR), the spleen GITR gene expression levels of the four groups were quantified. The results indicated that the percentage contents of CD4(+) CD25(+) regulatory T cells in CD4(+) T cells in peripheral blood were reduced in the F-treated groups. Compared with the control group, the GITR gene expression in mice spleen in the groups treated with 50, 100, and 150 mg/L NaF decreased by 15.6%, 42.5%, and 69.9%, respectively.

• 出版日期2017-1
• 单位山西农业大学; 菏泽学院