Population Structure of Portunus pelagicus in Coastal Areas of Malaysia Inferred from Microsatellites

作者：Chai Chuan Jian; Esa Yuzine Bin^*; Ismail Muhammad Fadhil Syukri; Kamarudin Mohd Salleh

来源：Zoological Studies, 2017, 56: 26.

DOI：10.6620/ZS.2017.56-26

• Portunus pelagicus
• Blue swimmer crabs
• Population genetic structure
• Microsatellites

Portunus pelagicus, distributed throughout the Indo-West Pacific region, is one of the large and edible species of blue swimmer crabs. Increasing demand for the frozen and canned crabmeat industry worldwide has now relied mainly on P. pelagicus which in turn generates splendid income for the fisherman communities. In the present study, the population genetic structure of P. pelagicus was examined using six pairs of microsatellite loci. A total of 87 crab samples were collected from five different coastal areas of Malaysia. Genomic DNA was extracted from each sample for polymerase chain reaction (PCR) amplification and fragment analysis. Four out of six microsatellite primers revealed polymorphic loci in P. pelagicus sampled. The number of alleles per locus in P. pelagicus ranged from 14 to 34. Microsatellites analyses indicated low levels of genetic differentiation among the P. pelagicus populations. The average observed heterozygosity (H-O = 0.48) obtained was lower than the standard heterozygosity found in most marine populations (H-O = 0.79). The high F-IS values (mean F-IS = 0.4756) and low F-ST values (mean F-ST = 0.0413) also suggested the existence of inbreeding among different populations of P. pelagicus. In conclusion, this study was able to shed light on the population structure of P. pelagicus in coastal areas of Malaysia.

• 出版日期2017