New photocleavable linker: alpha-Thioacetophenone-type linker

作者：Yonezawa Hirotaka; Nishiyama Yoshitake; Takeo Koji; Iwatsubo Takeshi; Tomita Taisuke; Yokoshima Satoshi; Fukuyama Tohru^*

来源：Bioorganic & Medicinal Chemistry Letters, 2014, 24(13): 2831-2833.

DOI：10.1016/j.bmcl.2014.04.104

• Cleavable linker
• Affinity purification
• Fluorescence spectroscopy
• Photolysis
• Norrish type II reaction

Photocleavable linkers are advantageous over the common linkers because they could be cleaved without using reagents. A novel photocleavable linker with an alpha-thioacetophenone moiety has been developed. This linker, which can be cleaved upon irradiation at 365 nm via the Norrish type II reaction, is applicable to a protein affinity purification system, allowing target proteins to be effectively isolated. This novel linker would serve as an effective tool in chemical biology.

• 出版日期2014-7-1