An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors

作者：Hisao Grant S; Harland Michael A; Brown Robert A; Berthold Deborah A; Wilson Thomas E; Rienstra Chad M^*

来源：Journal of Magnetic Resonance, 2016, 265: 172-176.

DOI：10.1016/j.jmr.2016.01.009

• Solid-state NMR
• Magic angle spinning
• MAS rotor-packing
• Gel phase samples

The study of mass-limited biological samples by magic angle spinning (MAS) solid-state NMR spectroscopy critically relies upon the high-yield transfer of material from a biological preparation into the MAS rotor. This issue is particularly important for maintaining biological activity and hydration of semi-solid samples such as membrane proteins in lipid bilayers, pharmaceutical formulations, microcrystalline proteins and protein fibrils. Here we present protocols and designs for rotor-packing devices specifically suited for packing hydrated samples into Pencil-style 1.6 mm, 3.2 mm standard, and 3.2 mm limited speed MAS rotors. The devices are modular and therefore readily adaptable to other rotor and/or ultracentrifugation tube geometries.

• 出版日期2016-4