A Low-density Antigen Array for Detection of Disease-associated Autoantibodies in Human Plasma

作者：Somiari Richard I^*; Sutphen Rebecca; Renganathan Kutralanathan; Russell Stephen; Pastwa Elzbieta; Somiari Stella A

来源：Cancer Genomics and Proteomics, 2016, 13(1): 13-19.

• Antigene array
• autoantibody detection
• autoimmune disease
• ovarian cancer
• plasma

Background/Aim: The ability to easily detect autoantibodies will help in the early diagnosis and treatment of certain diseases. Currently, available methods for autoantibody detection are time-consuming and cumbersome. The present study aimed to evaluate the performance of an easy-to-use antigen array developed for autoantibody detection. Materials and Methods: Plasma from 9 female donors diagnosed with ovarian cancer (test group) and 9 matched donors with no history of cancer (reference group) were screened and results were compared. Autoantibody levels >= 1.5-times the background were classified as positive. Results: A total of 29 autoantibodies were detected, out of which the autoantibody against osteoprotegerin was found to be significantly higher in the "test" group (p<0.001) while those against macrophage migration inhibitor factor, interleukin-2 and vascular endothelial growth factor were lower (p<0.05). Conclusion: The evaluated antigen array has potential as a simple method for determining the presence/absence of up to 90 disease-associated autoantibodies in a plasma specimen.

• 出版日期2016-2