Near-Infrared Spectroscopic Evaluation of Single-Kernel Deoxynivalenol Accumulation and Fusarium Head Blight Resistance Components in Wheat

作者：Peiris Kamaranga H S; Bockus William W; Dowell Floyd E^*

来源：Cereal Chemistry, 2016, 93(1): 25-31.

DOI：10.1094/CCHEM-03-15-0057-R

Fusarium head blight (FHB) symptoms, single-kernel deoxynivalenol (DON) levels, and distribution of DON levels among kernels in response to artificial FHB inoculation were investigated in three selected wheat cultivars that had different reported levels of FHB resistance. DON levels were estimated with near-infrared spectroscopy. The percentages of DON-containing spikelets per spike of 15.2, 49.7, and 89.1% were significantly different among point-inoculated spikes of Everest, Karl 92, and Overley, respectively. The percentage of visually Fusarium-damaged kernels in point-inoculated Karl 92 and Overley spikes was significantly higher than for Everest. However, the DON-containing spikelets per spike and visually Fusarium-damaged kernels values for spray-inoculated spikes were not significantly different among the three cultivars. In spray-inoculated spikes, DON levels in kernels ranged from 0 to 291.3 ppm, whereas the variation of DON levels in spikelet positions was random. In contrast, DON levels in spikelets below the inoculated spikelet in point-inoculated spikes showed marked differences among the three cultivars. Overley had the highest DON accumulation in kernels. This near-infrared spectroscopy method may be used as a novel way to evaluate wheat cultivars for FHB resistance to toxin accumulation. Other resistance components such as resistance to pathogen infection and resistance to pathogen spread may also be evaluated.

• 出版日期2016-2