A superoxide anion biosensor based on direct electron transfer of superoxide dismutase on sodium alginate sol-gel film and its application to monitoring of living cells

作者：Wang, Xiuhua; Han, Min; Bao, Jianchun; Tu, Wenwen; Dai, Zhihui^*

来源：Analytica Chimica Acta, 2012, 717: 61-66.

DOI：10.1016/j.aca.2011.12.045

• Superoxide dismutase
• Sodium alginate sol-gel film
• Living cells
• Superoxide sensor
• Direct electron transfer

The direct electron transfer of superoxide dismutase (SOD) was greatly facilitated by sodium alginate (SA) sol-gel film with the formal potential of 0.14 V, which was just located between O-2(center dot-)/O-2 and O-2(center dot-)/H2O2. The preparation of the SOD/SA modified electrode was simple without any mediators or promoters. Based on bimolecular recognition for specific reactivity of SOD/SA toward O-2(center dot-) the SOD modified electrode was utilized to measure O-2(center dot-) with good analytical performance, such as low applied potential (0 V), high selectivity (no obvious interference), wide linear range (0.44-229.88 mu M) and low detection limit (0.23 mu M) in pH 7.0 phosphate buffer solution. Furthermore, it could be successfully exploited for the determination of O-2(center dot-) released from living cells directly adhered on the modified electrode surface. Thus, the proposed O-2(center dot-) biosensor, combining with the properties of SA sol-gel film, provided a novel approach for protein immobilization, direct electron transfer study of the immobilized protein and real-time determination of O-2(center dot-) released from living cells.

• 出版日期2012-3-2
• 单位南京师范大学