Determination of mycotoxins in cereals by liquid chromatography tandem mass spectrometry

作者：Soleimany F; Jinap S^*; Abas F

来源：Food Chemistry, 2012, 130(4): 1055-1060.

DOI：10.1016/j.foodchem.2011.07.131

• Simultaneous determination
• Mycotoxin
• LC-MS/MS
• Validation
• Cereal

A liquid chromatography tandem mass spectrometry (LC-MS/MS) method is described for simultaneous determination of aflatoxins (AFB(1), AFB(2), AFG(1), and AFG(2)), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1 and FB2), T2 and HT2-toxin in cereals. One-step extraction using solvent mixtures of acetonitrile:water:acetic acid (79:20:1) without any clean-up was employed for extraction of these mycotoxins from cereals. The mean recoveries of mycotoxins in spiked cereals ranged from 76.8% to 108.4%. Limits of detection (LOD) and quantification (LOQ) ranged 0.01-20 and 0.02-40 ng/g, respectively. The developed method has been applied for the determination of mycotoxins in 100 cereal samples collected from Malaysian markets. A total of 77 cereal samples (77%) contaminated with at least one of these mycotoxins. Occurrence of mycotoxins in commercial cereal samples were 70%, 40%, 25%, 36%, 19%, 13%, 16, and 16% for aflatoxins, OTA, ZEA, DON, FB1, FB2, T2 and HT2-toxin, respectively. The results demonstrated that the procedure was suitable for the determination of mycotoxins in cereals and could be implemented for the routine analysis.

• 出版日期2012-2-15