科研之友
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摘要
Gastric cancer is the most common malignancy in digestive tract with rising incidence and younger trend. Cyclin-dependent kinase-like (CDKL1), a member of cell division cyclin (CDC-2) associated serine threonine protein kinase family, is found to be overexpressed in malignancy such as breast cancer and gastric cancer. The specific role and related mechanism in gastric cancer has not been reported. This study intends to investigate the impact of CDKL1 in gastric cancer cell growth, colony formation, and cell apoptosis. CDKL1 expression in human gastric cancer and adjacent tissues was tested by Western blot. CDKL1 level was regulated by lentivirus transfection. Cell proliferation was determined by MTT assay. Colony formation was evaluated by the colony forming assay. Cell cycle and apoptosis were detected by flow cytometry. CDKL1 significantly upregulated in gastric cancer tissue compared with adjacent tissue (P < 0.05). Downregulation of CDKL1 obviously restrained cell proliferation and colony formation, induced cell apoptosis, and blocked cell cycle in G1 phase. CDKL1 overexpression obtained the opposite results. CDKL1 was related to gastric cancer cell growth, colony formation, apoptosis, and cell cycle. It could be treated as a potential target for gastric cancer therapy.
- 出版日期2017
- 单位山西省肿瘤医院
