A disposable sampling device to collect volume-measured DBS directly from a fingerprick onto DBS paper

作者：Lenk Gabriel^*; Sandkvist Soren; Pohanka Anton; Stemme Goran; Beck Olof; Roxhed Niclas

来源：Bioanalysis, 2015, 7(16): 2085-2094.

DOI：10.4155/bio.15.134

Background: DBS samples collected from a fingerprick typically vary in volume and homogeneity and hence make an accurate quantitative analysis of DBS samples difficult. Results: We report a prototype which first defines a precise liquid volume and subsequently stores it to a conventional DBS matrix. Liquid volumes of 2.2 mu l +/- 7.1% (n = 21) for deionized water and 6.1 mu l +/- 8.8% (n = 15) for whole blood have been successfully metered and stored in DBS paper. Conclusion: The new method of collecting a defined volume of blood by DBS sampling has the potential to reduce assay bias for the quantitative evaluation of DBS samples while maintaining the simplicity of conventional DBS sampling.

• 出版日期2015