Activity of protein MalE (maltose-binding protein) fused to cytoplasmic and periplasmic regions of an Escherichia coli inner membrane protein

作者：Dassa E^*; Lambert P

来源：Research in Microbiology, 1997, 148(5): 389-395.

DOI：10.1016/S0923-2508(97)83869-7

• MalE protein
• MalG protein
• cytoplasmic membrane
• integral protein
• topology
• Escherichia coli
• fusion

We analysed the properties of mature MBP (maltose-binding protein or MaIE protein) fused to an integral cytoplasmic membrane protein of Escherichia coli. Fusion of MaIE to the first MaIG periplasmic loop enabled a strain defective in the maIE gene to utilize maltose. In contrast, fusion of MaIE to a cytoplasmic loop did not complement the maIE Delta 444 deletion. We obtained results highly correlated with those obtained by using alkaline phosphatase as a reporter for the topology of MaIG. We discuss the possibility of genetically determining the topology of cytoplasmic membrane proteins by a method based on engineered fusions to MBP.

• 出版日期1997-6