Novel ISCR1-linked resistance genes found in multidrug-resistant Gram-negative bacteria in southern China

作者：Wang, Fengping; Wu, Kuihai; Sun, Jingjing; Wang, Qian; Chen, Qing; Yu, Shouyi; Rui, Yongyu^*

来源：International Journal of Antimicrobial Agents, 2012, 40(5): 404-408.

DOI：10.1016/j.ijantimicag.2012.06.016

• Orf513
• ABC transporter
• Quinolone resistance

Non-duplicate multidrug-resistant (MDR) Gram-negative bacteria (n = 1329) isolated from southern China between January 2008 and December 2009 were investigated for the presence of ISCR1 as well as characterisation of ISCR1-linked resistance genes. Of 433 ISCR1-positive strains, 151 appeared to carry ISCR1-linked resistance genes. Seven different ISCR1-linked resistance gene arrays were identified by restriction fragment length polymorphism (RFLP) and DNA sequencing analysis. Many of these arrays are reported in some species for the first time. A total of 12 genes, including a novel ABC transporter (GenBank accession no. GU944725), qnrA1, qnrB2, qnrB6, bla(DHA-1), ampR, bla(CTX-M-9), bla(PER-1), insB, sapA-like peptide transport periplasmic protein, putative glutathione S-transferase and short- chain dehydrogenase/ reductase, were detected. This study was the first to employ PCR-RFLP using HinfI and RsaI to analyse ISCR1-linked genes. ISCR1 was widely disseminated among MDR Gram-negative bacteria and was in close association with quinolone resistance and beta-lactamase genes (class A and class C) in southern China.

• 出版日期2012-11
• 单位南方医科大学