Multiple Compounds Determination and Fingerprint Analysis of Agrimonia pilosa Ledeb by High-Performance Liquid Chromatography

作者：Wang Y; Yin L; Lv G; Xu Y; Xu L; Qi Y; Zheng L^*; Peng J

来源：Acta Chromatographica, 2014, 26(1): 137-156.

DOI：10.1556/AChrom.26.2014.1.11

• Agrimonia pilosa Ledeb
• HPLC-DAD
• hierarchical cluster analysis
• quality control
• fingerprint analysis

In the present paper, a simple and reliable high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed both for quantitative determination and fingerprint analysis of Agrimonia pilosa Ledeb for quality control. Under the optimized HPLC conditions, seven bioactive compounds including rutin, quercetin-3-rhamnoside, luteoloside, tiliroside, apigenin, kaempferol, and agrimonolide were determined simultaneously. For fingerprint analysis, 11 common peaks were selected as the characteristic peaks to evaluate the similarities of 16 different samples collected from different origins in China. Besides, hierarchical cluster analysis (HCA) was also performed to evaluate the variation of the raw materials. This is the first report of using a simple method for quality control of A. pilosa Ledeb through multi-component determination and chromatographic fingerprint analysis to the best of our knowledge.

• 出版日期2014-3
• 单位大连医科大学