Apolipoprotein A-IV as a novel gene associated with polycystic ovary syndrome

作者：Kim Yong Soo; Gu Bon Hee; Choi Bum Chae; Kim Myung Sun; Song Sangjin; Yun Ji Hyun; Chung Mi Kyung; Choi Cheol Hee; Baek Kwang Hyun^*

来源：International Journal of Molecular Medicine, 2013, 31(3): 707-716.

DOI：10.3892/ijmm.2013.1250

• apolipoprotein A-IV
• follicular fluid
• single nucleotide polymorphism
• two-dimensional gel electrophoresis

Polycystic ovary syndrome (PCOS) is a common endocrine-metabolic disorder, affecting 6-10% of women of reproductive age. The etiology remains poorly understood. To investigate the differentially expressed proteins from PCOS patients versus healthy women, the protein expression in follicular fluid was analyzed using two-dimensional electrophoresis (2-DE). Since follicular fluid contains a number of secretory proteins required for oocyte fertilization and follicle maturation, it is possible that follicular fluid can be used as a provisional source for identifying pivotal proteins associated with PCOS. In this study, six overexpressed proteins [kininogen 1, cytokeratin 9, antithrombin, fibrinogen gamma-chain, apolipoprotein A-IV (apoA-IV) precursor and alpha-1-B-glycoprotein (A1BG)] in follicular fluids from PCOS patients were identified with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) and nano LC-MS/MS. Western blot analysis confirmed that the protein expression levels of apoA-IV precursor and A1BG were increased in follicular fluid from PCOS patients compared with those from normal controls. The analysis of protein expression for other proteins revealed individual variation. These results facilitate the understanding of the molecular mechanisms of PCOS and provide candidate biomarkers for the development of diagnostic and therapeutic tools.

• 出版日期2013-3