科研之友
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摘要
Caspase-3 activation was analyzed inside single living cells during bufalin-induced human lung adenocarcinoma (ASTC-a-1) cell apoptosis by using multi-photon excitation spectra and fluorescence lifetime imaging microscopy (FLIM). CCK-8 was used to assay the inhibition of bufalin on the cells'viability. The dynamical emission spectra of SCAT3 which is a fluorescence resonance energy transfer (FRET) plasmid, and fluorescence lifetime of ECFP were performed inside single living cells, stably expressing SCAT3 after bufalin treatment. Compared with controls, SCAT3 did not change after 24 h, but was cleaved after 48 h, which were verified by the changes of fluorescence spectra of SCAT3 and fluorescence lifetime of ECFP. Our data shows that the cells'growth is significantly inhibited by bufalin in a dose-dependent manner;caspase-3 is involved in the bufalin-induced cell death.
