Newcastle disease virus-like particles induce dendritic cell maturation and enhance viral-specific immune response

作者：Qian, Jing; Ding, Jiaxin; Yin, Renfu; Sun, Yixue; Xue, Cong; Xu, Xiaohong; Wang, Jianzhong; Ding, Chan; Yu, Shengqing; Liu, Xiufan; Hu, Shunlin; Cong, Yanlong^*; Ding, Zhuang^*

来源：Virus Genes, 2017, 53(4): 555-564.

DOI：10.1007/s11262-017-1451-1

• Newcastle disease virus
• Virus-like particle
• Dendritic cell
• Immune response

Circulating of genotype VII Newcastle disease virus (NDV) is a great threat to the poultry industry worldwide. Virus-like particles (VLPs) are increasingly being considered as potential viral vaccines due to their safety and efficacy. In this study, we analyzed in vitro the stimulatory effects of VLPs containing the matrix and hemagglutinin-neuraminidase of genotype VII NDV on dendritic cells (DCs) and evaluated their immunogenicity in mice. The results showed that immature bone marrow-derived dendritic cells (BMDCs) responded to stimulation with VLPs by up-regulating expressions of MHC II, CD40, CD80, and CD86 molecules and by increasing the cytokine secretions of TNF-alpha, IFN-gamma, IL-6, and IL-12p70. Besides, VLPs enhanced the immunostimulatory capacity of DCs to stimulate autologous T cell proliferation. Furthermore, VLPs can induce efficient humoral and cellular immune responses, and recruit mature DCs to the spleen in C57BL/6 mice, as shown by an obvious increase in double-positive proliferation of splenic CD11c+CD86+ cells. These data indicate that NDV VLPs can be a valuable candidate for NDV vaccine development.

• 出版日期2017-8
• 单位吉林大学; 中国农业科学院上海兽医研究所; 扬州大学; 吉林农业大学