Astragalin suppresses inflammatory responses via down-regulation of NF-kappa B signaling pathway in lipopolysaccharide-induced mastitis in a murine model

作者：Li Fengyang; Liang Dejie; Yang Zhengtao; Wang Tiancheng; Wang Wei; Song Xiaojing; Guo Mengyao; Zhou Ershun; Li Depeng; Cao Yongguo^*; Zhang Naisheng

来源：International Immunopharmacology, 2013, 17(2): 478-482.

DOI：10.1016/j.intimp2013.07.010

• Astragalin
• Lipopolysaccharide (LPS)
• Mastitis
• Cytokine
• Nuclear factor-kappaB (NF-kappa B)

Mastitis is a prevalent and economic disease around the world and defined as infection and inflammation of the mammary gland. Astragalin, a bioactive component isolated from persimmon or Rosa agrestis, has been reported to have anti-inflammatory properties. To investigate the potential therapeutic effect of astragalin in mastitis, a murine model of mastitis was induced by administration of LPS in mammary gland. Astragalin was applied 1 h before and 12 h after LPS treatment. The results showed that astragalin attenuated the infiltration of inflammatory cells, the activity of myeloperoxidase (MPO) and the expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) in a dose-dependent manner. Additionally, Western blotting results showed that astragalin efficiently blunt decreased nuclear factor-kappaB (NF-kappa B) activation by inhibiting the degradation and phosphorylation of I kappa B alpha and the nuclear translocation of p65. These results suggested that astragalin exerts anti-inflammatory properties in LPS-mediated mastitis, possibly through inhibiting inhibition of the NF-kappa B signaling pathway, which mediates the expression of pro-inflammatory cytokines. Astragalin may be a potential therapeutic agent against mastitis.

• 出版日期2013-10
• 单位吉林大学