Manufacture of Concentrated, Lipid-based Oxygen Microbubble Emulsions by High Shear Homogenization and Serial Concentration

作者：Thomson Lindsay M; Polizzotti Brian D; McGowan Frances X; Kheir John N^*

来源：Jove-Journal of Visualized Experiments, 2014, (87): e51467.

DOI：10.3791/51467

• Bioengineering
• Issue 87
• oxygen
• microparticles
• microbubbles
• homogenization
• encapsulation
• lipid monolayer
• drug delivery

Gas-filled microbubbles have been developed as ultrasound contrast and drug delivery agents. Microbubbles can be produced by processing surfactants using sonication, mechanical agitation, microfluidic devices, or homogenization. Recently, lipid-based oxygen microbubbles (LOMs) have been designed to deliver oxygen intravenously during medical emergencies, reversing life-threatening hypoxemia, and preventing subsequent organ injury, cardiac arrest, and death. We present methods for scaled-up production of highly oxygenated microbubbles using a closed-loop high-shear homogenizer. The process can produce 2 L of concentrated LOMs (90% by volume) in 90 min. Resulting bubbles have a mean diameter of similar to 2 mu m, and a rheologic profile consistent with that of blood when diluted to 60 volume %. This technique produces LOMs in high capacity and with high oxygen purity, suggesting that this technique may be useful for translational research labs.

• 出版日期2014-5