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摘要
A system of sequential extraction and cleaning coupled with high performance liquid chromatography (HPLC) by multi-wavelength detection applied to the analysis of 46 synthetic pigments and dyes in food was established. The system of sequential extraction was composed of three grades. In the first grade of extraction,the Sudan dyes were extracted with n-hexane and cleaned effectively by gel permeation chromatography. The recoveries of 8 Sudan dyes were not less than 80%. In the second grade of extraction,most of water-soluble synthetic pigments were extracted with water assisted by acetonitrile. And the recoveries were not less than 60%. In the third grade of extraction,a few of compounds were extracted using methanol and ammonia water whose concentration was 1.0%. And the recoveries were not less than 55%. It was unnecessary to clean up the extract after the second and third grade of extraction. The detection results of samples from markets showed that the system of sequential extraction and cleaning was suitable for the food stuffs contained protein and fat.
- 出版日期2012-2
- 单位江苏大学; 中国检验检疫科学研究院
