科研之友
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摘要
A new chromatographic and densitometric method has been established for quantitative analysis of lumefantrine and artemether present together in combined tablet to treat malaria. The method employed TLC aluminum plate precoated with silica gel 60F(254) as the stationary phase. The solvent system consisted of toluene:ethyl acetate:acetic acid (2:7.5:0.5, v/v/v) as the mobile phase. Densitometric analysis was carried out in the reflectance mode at 269nm for lumefantrine and 519nm for artemether. The method is specific for analyte constituents examined and characterized by high sensitivity. The LOD and LOQ of lumefantrine were 7.32 and 26.15ng; for artemether, 2.43 and 6.12ng per band, respectively. Recovery from 98.40% to 99.45% for lumefantrine and 100.09% to 101.61% for artemether and linear range from 60 to 360ng for lumefantrine and 10 to 60ng for artemether per band. The method was characterized by good precision with RSD from 0.11 to 1.41.
- 出版日期2014-6-15
