Antidepressive-Like Property of Dichloromethane Fraction of Pimenta pseudocaryophyllus and Relevance of Monoamine Metabolic Enzymes

作者：Fajemiroye James Oluwagbamigbe; Rodrigues Martins Jose Luis; Ghedini Paulo Cesar; Galdino Pablinny Morreira; Marciano de Paula Joelma Abadia; de Paula Jose Realino; Da Rocha Fabio Fagundes; Costa Elson Alves^*

来源：Evidence-Based Complementary and Alternative Medicine, 2013, 2013: 659391.

DOI：10.1155/2013/659391

Pimenta pseudocaryophyllus popularly referred to as craveiro is considered as a calming agent in different local preparations.. e present study attempted to examine antidepressant-like effect of dichloromethane fraction (DF) and role of monoamine oxidase (MAO), tryptophan, and tyrosine hydroxylase. Based on the research focus, tail suspension (TS), forced swimming (FS), and open field (OF) tests were conducted after oral administration of DF (125, 250, or 500 mg/Kg). Ex vivo assay of MAO was also conducted to evaluate inhibitory effect of DF (250 mg/Kg). Administration of DF elicits antidepressant-like response in the TS and FS. However, DF 500 mg/Kg did not alter mice performance in these models.. e data obtained in the OF showed a reduction in total crossing and rearing activity; these effects suggest motor interference in TS and FS performance. Mice pretreatment with p-chlorophenylalanine methyl ester (PCPA) (100 mg/kg, i.p.-serotonin biosynthesis inhibitor) for 4 consecutive days or acute administration of alpha-methyl-p-tyrosine (AMPT) (100 mg/kg, i.p.-catecholamine synthesis inhibitor) blocked anti-immobility effect of DF in the FS. In ex vivo assay of MAO, DF did not inhibit catabolic activity of MAO. Our findings support antidepressant-like activity of DF and suggest an effect that depends on monoamine biosynthesis.

• 出版日期2013