Analysis of the IKK beta/NF-kappa B Signaling Pathway During Embryonic Angiogenesis

作者：Hou Yanjun; Li Fu; Karin Michael; Ostrowski Michael C^*

来源：Developmental Dynamics, 2008, 237(10): 2926-2935.

DOI：10.1002/dvdy.21723

• IKK (I kappa B kinase)
• angiogenesis
• development

The nuclear factor-kappa B (NF-kappa B) signaling pathway regulates cellular growth, survival, differentiation and development. In this study, the functions of I kappa B kinase (IKK)beta in angiogenesis during mouse development were examined. Conditional disruption of the Ikk beta locus in endothelial cells using the well-characterized Tie2-Cre transgene resulted in embryonic lethality between embryonic day (E) 13.5 and E15.5. Examination of the mutant embryos revealed that while deletion of Ikk beta occurred in endothelial cells throughout the embryo, only the vascular network in the fetal liver was affected. Disruption of the fetal liver vasculature was accompanied by decreased cell proliferation and increased apoptosis of hepatocytes, but hematopoiesis was not affected. Increased apoptosis was not observed outside of fetal liver in the mutant embryos. These results indicate that the IKK beta/NF-kappa B pathway plays a previously unappreciated role in development of the sinusoidal vasculature in the fetal liver and additionally that this pathway is critical in the crosstalk between endothelial cells and hepatocytes during mouse development. Developmental Dynamics 237. 2926-2935, 2008.

• 出版日期2008-10