A lipid based multi-compartmental system: Liposomes-in-double emulsion for oral vaccine delivery

作者：Liau Jin Jau; Hook Sarah; Prestidge Clive A; Barnes Timothy J^*

来源：European Journal of Pharmaceutics and Biopharmaceutics, 2015, 97: 15-21.

DOI：10.1016/j.ejpb.2015.09.018

• Liposome
• Emulsion
• Oral
• Multi-compartment
• Vaccine
• In vitro

The gastric mucosa provides the entry point for the majority of pathogens, as well as being the induction site for protective immunity; however, there remain few examples of oral vaccines due to the challenges presented by the gastrointestinal route. In this study, we develop a lipid-based multi-compartmental system for oral vaccine delivery. Specifically, we have optimised the formulation of a water-in-oil-in-water double emulsion prepared from a triglyceride - soya bean oil, using surfactants Span 80/Tween 80 and Pluronic F127 to stabilise the internal and external water phases, respectively. Into the internal water phase, we also incorporated a PEGylated liposome, prepared using hydrogenated phosphatidyl choline as a carrier for our model protein, FITC-labelled ovalbumin. We demonstrated the successful incorporation of intact liposomes into the internal water phase of the double emulsion using imaging techniques including cryo-SEM and confocal microscopy. Finally, we use in vitro release studies of FITC-ovalbumin, to provide further confirmation of the multi-compartmental structure of the double emulsion system and demonstrate significant extended release of the entrapped model antigen compared with PEG-liposomes; these characteristics are attractive for oral vaccine delivery.

• 出版日期2015-11