Differential Binding of Active and Inactive Integrin to Talin

作者：Wang, Dongchuan; Guo, Qiang; Wei, Ailin; Li, Ang^*

来源：Protein Journal, 2018, 37(3): 280-289.

DOI：10.1007/s10930-018-9776-8

• Integrin
• Talin
• Active integrin
• Integrin signaling
• Outside-in signaling
• Integrin binding

Bi-directional signaling of integrins plays an important role in platelet and leukocyte function. Talin plays a key role in integrin bi-directional signaling and its binding to integrin is highly regulated. The precise regulation of the recruitment and binding of talin to integrin is still being elucidated. In particular, the recruitment of talin to integrin is controlled by the RAP-1 and RIAM/lamellipodin signaling axis and the affinity between talin and integrin is regulated by the conformation or protease cleavage of talin. However, whether the binding between integrin and talin is also regulated by integrin conformation has not been thoroughly explored before. In this work, we used biochemical binding assays to study the potential role of integrin conformational changes in integrin-talin interactions. Constitutively active integrin alpha IIbb3 binds markedly stronger to talin than inactive alpha IIbb3. Inactive alpha IIbb3 markedly increases its binding to talin once activated, regardless of how alpha IIbb3 is activated. Further, the increased binding to talin is b3 tail dependent. Our results suggest that integrin conformation is another regulatory mechanism for integrin-talin interaction.

• 出版日期2018-6
• 单位四川大学