ABCG5/G8 polymorphisms and markers of cholesterol metabolism: systematic review and meta-analysis

作者：Jakulj Lily^*; Vissers Maud N; Tanck Michael W T; Hutten Barbara A; Stellaard Frans; Kastelein John J P; Dallinga Thie Geesje M

来源：The Journal of Lipid Research, 2010, 51(10): 3016-3023.

DOI：10.1194/jlr.M008128

• polymorphism
• LDL-cholesterol
• plant sterol

Genetic variation at the ABCG5/G8 locus has been associated with markers of cholesterol homeostasis. As data originate from small-scale studies, we performed a meta-analysis to study these associations in a large dataset. We first investigated associations between five common ABCG5/G8 polymorphisms (p.Q604E, p.D19H, p.Y54C, p.T400K, and p.A632V) and plasma sterol levels in 245 hypercholesterolaemic individuals. No significant associations were found. Subsequently, our data were pooled into a meta-analysis that comprised 3,364 subjects from 16 studies (weighted mean age, 46.7 +/- 10.5 years; BMI, 23.9 +/- 3.5 kg/m(2)). Presence of the minor 632V allele correlated with reduced LDL-C concentrations (n = 367) compared with homozygosity for the 632A variant [n = 614; -0.11 mmol/l (95% CI, range: -0.20 to -0.02 mmol/l); P = 0.01]. The remaining polymorphisms were not associated with plasma lipid levels. Carriers of the 19H allele exhibited lower campesterol/TC (n = 83; P < 0.001), sitosterol/TC (P < 0.00001), and cholestanol/TC (P < 0.00001), and increased lathosterol/TC ratios (P = 0.001) compared with homozygous 19D allele carriers (n = 591). The ABCG8 632V variant was associated with a clinically irrelevant LDL-C reduction, whereas the 19H allele correlated with decreased cholesterol absorption and increased synthesis without affecting the lipid profile. Hence, associations between frequently studied missense ABCG5/G8 polymorphisms and markers of cholesterol homeostasis are modest at best. Jakulj, L., M. N. Vissers, M. W. T. Tanck, B. A. Hutten, F. Stellaard, J.J.P. Kastelein, and G. M. Dallinga-Thie. ABCG5/G8 polymorphisms and markers of cholesterol metabolism: systematic review and meta-analysis. J. Lipid Res. 2010. 51: 3016-3023.

• 出版日期2010-10