Neolitsea sericea Essential Oil Attenuates LPS-induced Inflammation in RAW 264.7 Macrophages by Suppressing NF-kappa B and MAPK Activation

作者：Yoon Weon Jong; Moon Ji Young; Kang Ji Yong; Kim Gi Ok; Lee Nam Ho^*; Hyun Chang Gu

来源：Natural Product Communications, 2010, 5(8): 1311-1316.

• Chemical composition
• essential oil
• inflammation
• Neolitsea sericea
• nuclear factor-kappa B

The chemical composition and anti-inflammatory activities of hydrodistilled essential oil from Neolitsea sericea leaves (NSE) have been investigated for the first time. The chemical constituents of NSE were analysed by GC-MS and found to include sericenine (32.3%), sabinene (21.0%), trans-beta-ocimene (13.3%), beta-caryophyllene (4.8%), and 4-terpineol (4.2%). The effects of NSE on nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumour necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6 production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages were also examined. Pro-inflammatory cytokine and mediator tests indicated that NSE has excellent dose-dependent inhibitory activities. To further examine the mechanism responsible for the inhibition of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression by NSE, we examined the effect of NSE on nuclear factor-kappa B (NF-kappa B) activation and the phosphorylation of mitogen-activated protein kinases (MAPK). NSE inhibited NF-kappa B activation by LPS, and this was associated with the abrogation of I kappa B-alpha phosphorylation and subsequent decreases in nuclear p50 and p65 protein levels. Further, the phosphorylation of p38, ERK and JNK was suppressed by NSE in a concentration-dependent manner. These results suggest that NSE exerts anti-inflammatory effects in LPS-stimulated RAW 264.7 macrophages by inhibition of NF-kappa B activation and MAPK phosphorylation, and, therefore, may be useful for treatment of inflammatory diseases.

• 出版日期2010-8